The point-of-use disposable bag testing is a procedure equipped to validate reliability and reproducibility of methodology used in pressure decay. The high growth in terms of value for media and time interval required to perform a cell culture process that demands a strict examination during the test. In a GMP facility bioreactor leakage may generate economic loss and affect the scheduled production. The point-in-use disposable bag testing is useful to quantify the risk in single-use production facilities. In a bioreactor including the tubing system for detecting leakages that have been introduced by the operator the pre-use disposable bag testing can be used.